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Materials and Methods Ethics Statement This study was carried out in strict accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals of the Brazilian National Council of Animal Experimentation ( and the NIH Guidelines for the Care and Use of Laboratory Animals

Follow-up pairwise comparisons revealed that 1-MX differed from the control group for all outcomes

Assays of methylenetetrahydrofolate reductase and methionine synthase activities by monitoring 5-methyltetrahydrofolate and tetrahydrofolate using high-performance liquid chromatography with fluorescence detection

Following the generation of large amounts of ROS, necroptosis is activated