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Transfection mixes were prepared using 0.25 nM siRNA and 2.5 l Lipofectamine RNAiMAX Transfection Reagent (Cat

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5.2 Generational impact analysis The reproductive consequences of EED exposure demonstrate remarkable persistence, extending beyond the directly exposed (F0) generation to affect subsequent offspring (F1-F3) through distinct but interconnected mechanisms

In fact, GSH is essential for some functions of the immune system, both innate and adaptive, including T-lymphocyte proliferation, 38 , 39 phagocytic activity of polymorphonuclear neutrophils (PMN), 40 and dendritic cell functions, 41 and is also important for the first step of adaptive immunity, consisting of the antigen presentation by antigen-presenting cells (APC)