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iTRAQ samples were tryptically digested by using a FASP Protein Digestion Kit (Protein Discovery, San Diego, CA) according to the manufacturers instructions with slight modifications (Supplemental Data), samples were desalted by C18 solid-phase extraction (SPE) before isobaric labeling (SUPELCO)

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Moreover, nicotine metabolites, exerting effects similar to the activity of toxins produced by periopathogens, lead to the accumulation of free radicals and boost OS in periodontal tissues