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Cells were treated with MRTX1133 (a selective KRAS-G12D inhibitor, Cayman Chemical) at different concentrations for three hours before use in the KRAS In-well Lysis ELISA Kit protocol, with four replicates each

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Following this characterization, the organoids were dissociated, and the enriched AEC2 cells were seeded onto an OoC system, enabling their application in a microfluidic environment