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Frizzled-8 (FZD8) as a Wnt receptor is known to be necessary for the interaction between the Wnt/-catenin axis and c-Met since it is upregulated through the ERK/c-Fos cascade by c-Met in cancer stem-like cells [106]

Dosage a bit much, after adjusting to 1/2 now tolerable

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After overnight liquid culture, cells are plated in methocult GF+ H4435, Stem Cell Technologies) supplemented with cytokines (IL-3, IL-6, IL-7, G-CSF, GM-CSF, CF, Flt3 ligand, and erythropoietin) and various concentrations of known chemotherapeutic agents in combination with either compounds of the present disclosure