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The DM was analyzed by using the freeze-drying method (FreeZone bench top freeze dryer, VWR International bvba, B-3001 Leuven, Belgium)

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About Umbrella Labs Umbrella Labs is a U.S.-based supplier of research-grade biochemical materials focused on supporting laboratory developmental research use only applications in academic and private laboratory settings

The percentage of load-bearing was calculated as follows: Quantitative real-time polymerase chain reaction (RT-PCR) The cartilage-related gene expression in all groups was investigated by RT-PCR