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The samples were protected from sunlight and kept in the glove box under N 2 atmosphere (25 2 C) for 12 hours, until the reactions in all the samples had reached equilibrium

However, it is important to note that lipotropic injections do not target specific areas of fat, and they are not a quick or magic solution for weight loss

475 It has been proposed that due to the high Zn II binding affinity to the primary site, only the auxiliary site would be filled with Fe II in vivo with the other bound by a noncognate metal, most likely Zn II

The supernatant, containing the extracted metabolites, was transferred to a 384-well polypropylene plate and diluted in 35:65 methanol:water for positive mode analysis or 75:25 methanol:water for negative mode analysis