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In a 2003 in vitro experiment by Khavinson and colleagues, addition of micromolar concentrations of the peptide to cultured human somatic fibroblasts produced a measurable increase in telomerase reverse transcriptase activity and a corresponding extension of telomere length across 1020 passages, with cell cultures continuing to divide beyond the Hayflick limit observed in untreated controls

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Several critical questions remain unresolved: (1) most existing studies are confined to animal and cellular models, with a notable lack of large-scale population-based investigations and clinical validation, thereby impeding translational application

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