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The relationship between excessive iodine during pregnancy and adverse pregnancy complications

DNMT activity assay In accordance with the manufacturers instructions, we extracted nuclear proteins with an Active Motif Nuclear Extraction Kit (Active Motif, Carlsbad, USA) and evaluated DNMT activity with an EpiQui DNA Methyltransferase Activity/Inhibition Assay Kit (Epigentek, Farmingdale, USA) as reported previously 64

In nonbiological samples, Craig and Bartlett previously observed biphasic decay of MeHg in the presence of H 2 S in an aqueous reaction mixture, with an initial rapid decay and then a slow decay that were probably due to the formation of (MeHg) 2 S, which is a somewhat water-insoluble material, and evolution of DMeHg from the reaction mixture, respectively 27 , thus supporting the results of our cell-free study