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Media and culture conditions Wild-type yeast strains were pre-cultured in 5 mL of YP20D medium containing 10 g/L yeast extract, 20 g/L Bacto Peptone, and 20 g/L glucose at 30 C and 250 rpm for 48 h

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Immunoblot with antibody against lipoic acid in the indicated Jurkat SLC25A39 knockout cells expressing a vector control or SLC25A39 cDNA transduced with the indicated sgRNA