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Then, 50 L of BCA working reagent was added to 1 mL of diluted Lf-conjugated LPs and incubated for 30 min at 37 C

Figure 8 3.6 Modulation of hepatic lipid metabolites in bullfrogs by glutathione mix feeding 3.6.1 Sample raw data and principal component analysis As can be seen from Figure 9 , the samples of NC, FI and GSH groups were all in the 95% confidence interval, and could be spatially separated evenly without overlap, indicating that there were obvious differences in metabolites among the three groups, and they were able to be analyzed in the next step