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Thus, the optimal experimental conditions for this strategy were determined to be 30 , using PBS buffer at pH 6.5 for a reaction time of 20 min

In some embodiments, a pH of between about 6.0 and about 7.6, between about 7 and about 7.8, between about 7.0 and about 7.6, between about 7.2 and 7.6, between about 7.2 and about 7.4 is suitable for sterilization (e.g., by filtration or aseptic mixing or heat treatment and/or autoclaving (e.g., terminal sterilization)) of formulations or compositions described herein

Collagen synthesis: Improves structural integrity of connective tissues