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It is essential to collect the venom without impurities from pollen, honey, or other colony byproducts to maintain its purity and efficacy (Essam Elenany, 2024)

Nevertheless, to minimize any confounding effects from solvent interactions, the concentration of DMSO, known to disrupt membrane integrity, was maintained below 1% in all working solutions, in accordance with the guidelines of the Clinical and Laboratory Standards Institute and previous findings by Tuner et al