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For example, it has been shown that an increase in GSSG levels is required for the activation of defense signaling in response to infections by powdery mildews and bacteria (Vanacker et al., 2000

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Following 30 min of incubation at 4 C, extracts were cleared by centrifugation (17,000 g , 20 min, 4 C) and then supernatants were analysed by liquid chromatography and mass spectrometry (LCMS)