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Melatonin also reduced DNA damage from charged particles: plasmids in solution with melatonin treated with iron ions showed a 41% reduction in DSBs compared to controls [39]

STAR580 and STAR635P fluorophores were excited using a 561 nm and 640 nm laser line, depleted using 775 nm laser line and the emission was detected in the 605625 nm and 650720 nm range, respectively, in the STED mode

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2) Resuspend the cells in 10 mL of Equilibration buffer (LE buffer) with appropriate amount of PMSF (Final working concentration can be 1mM) and add lysozyme (Final working concentration is 0.2-0.4 mg/mL), or other protease inhibitors cocktail added