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Biofilm formation is a constant concern in hospitals as it is a major cause of hospital acquired infections (nosocomial infections)

Blood glucose was determined using a handheld glucometer (OneTouch Ultra Easy, LifeScan) following an overnight fast, and plasma insulin levels were quantified by ELISA according to the manufacturers protocol (Abcam)

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Individuals in the high-carbohydrate group had no restrictions on the form of carbohydrates consumed, while the low-carbohydrate group aimed for up to 20 g of carbohydrates daily, exclusively from vegetables, with restrictions on fruits, grain products, and sweets