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All descriptions refer to non-clinical research context only

After slices were dehydrated in progressively stronger alcohols, cleaned in xylene, and mounted, hematoxylin was used as a counterstain ( Measurement of the percentage area of a positive SIRT -1 immunoreaction in hepatic tissue Images were taken using a digital camera (Toucan type BX53, Japan) linked to a computer and a light microscope (Olympus model BX53, Tokyo, Japan)

The main limitations are reflected in the following aspects: 6.5.1 Lack of a specific in vivo biomarker system Currently, no biomarker profile has been established that can specifically and dynamically reflect the ferroptosis process in vivo

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