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Mitochondrial glutathione (GSH) plays indispensable roles in maintaining normal cellular functions, including protection against oxidative stress, as well as cell proliferation and division

For LC-MS analysis, samples were thawed on ice-water bath, then 40 L of whole blood was injected into 450 L cold extraction solvent comprising of mixture of Acetonitrile:Methanol:Milli-Q water in ratio 40:40:20 according to protocol described in Presterud et al 35

On the first day of training the animal was placed on the pedestal for 30 s prior to the first trial

This dynamic is closely tied to the peptide reserve theory, which suggests that maintaining a steady pool of specific signaling peptides in the system allows tissues to optimize cellular repair and adapt to physical stress more efficiently