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Western blot Cells were lysed in 1 RIPA buffer (Thermo Scientific, 89900) supplemented with cOmplete mini EDTA-free protease inhibitor tablets (Roche, 04693159001) and PhosSTOP phosphatase inhibitor tablets (Roche, 04906837001) and incubated on ice for 10 min

Helps the liver detoxify fat before bile is emitted, which takes stress off of the gallbladder

The data suggested that GHK may preferentially interact with hydroxyl and peroxyl radicals, while exhibiting more limited activity toward superoxide-related species

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