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The nuclear and cytoplasmic separation was performed by using NE-PER extraction kit (#78833 ThermoFisher)

In sleep deprivation studies, high dose creatine improved cognitive performance even compared to rested baselines She travels with creatine and bumps to 20 to 25 grams when she knows she is going to be sleep deprived Also being researched: mild cognitive impairment, early Alzheimers, TBI recovery in children

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A 2020 study 28 also showed that citicoline improves psychomotor vigilance, arousal, and visual working memory while also lowering oxidative stress compared with a placebo