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Ultrathin sections (50 70 nm) were obtained using an ultramicrotome (EM UC7, Leica, Wetzlar, Germany), double-stained with uranyl acetate and lead citrate, and observed under a transmission electron microscope (JEM 1200EX-II, JEOL, Tokyo, Japan)

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I made a commitment to myself to quit smoking during my vacation between jobs, so I decided to watch this in the best environment (at least most feasibly): on my laptop, parked at the top of the Columbia River Gorge, chain-smoking the remainder of my quit pack (a.k.a