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Blood differential counts Blood differential cell counts were carried out on fresh whole blood collected in EDTA rather than heparin tubes to avoid heparin-induced aggregation of platelets 77 , using a Coulter AcT diff2 Hematology Analyzer (Beckman-Coulter Corp., Miami, FL)

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The functional connectivity (FC) between the nucleus accumbens (NAcc), the ventral tegmental area (VTA), and the insula was calculated at ROI-wise level