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Liquid chromatography mass spectrometry was used to determine serum levels of nicotine

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[15] Cigarettes filters were originally made of cork and used to prevent tobacco flakes from getting on the smoker's tongue

Thus, these individuals presumably would not have had any change in potential CO protection or ANA prevalence between Periods 1 and 2, since vaping did not begin until 2007, but would have had a decrease in potential CO protection and, consistent with our hypothesis, a corresponding increase in ANA prevalence between Periods 2 and 3, as some of them took up vaping