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Directly, the aerosolized vapours from your handheld device come into contact with moist tissues and absorb water, which leads to dryness in the mouth and throat

RNA Isolation and Quantitative Reverse-Transcription (qRT)-PCR Analysis Total RNA was isolated from 100 mg of tobacco leaves using the TriPure reagent (SigmaAldrich), according to the manufacturers instructions, and reverse-transcribed with the MMLV enzyme (Invitrogen) and oligo(dT) 12-18