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A Centers for Disease Control and Prevention study of the 40 most widely used popular brands of moist snuff showed that the amount of nicotine per gram of tobacco ranged from 4.4 milligrams to 25.0 milligrams (3)

Several studies have reported physiological and molecular responses of tobacco to B deficiency, e.g., decline in nitrate content and nitrate reductase (NR) activity, increment in carbohydrate content, accumulation of chlorogenic acid, caffeoyl polyamine conjugates and phenylpropanoids, increment in phenylalanine ammonia-lyase (PAL) and polyphenoloxidase (PPO) activities in leaves [24,25,26], increment in putrescine levels in tobacco plants [27], and up-regulation of an asparagine synthetase ( AS ) gene and the glutamate dehydrogenase ( GDH ) genes in roots [28, 29]

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