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In order to investigate the reinstatement of drug seeking behavior, rodents are allowed to self-administer a drug of abuse and then after a specific amount of time (mostly about 14 days) drug self-administration is extinguished by withholding the drug

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16 Nicotine and other components of cigarette smoke induce microcirculation alterations with consequent vasoconstriction and hypoxyemia 17 , 18 and exhibit an inhibitory effect on the chemotaxis of neutrophils and lymphocytes