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The application of CRISPR/Cas9 genome editing in tobacco has demonstrated potential, for example, by knocking out nicotine biosynthesis genes to produce nicotine-free plants and targeting photosynthesis-related genes like Rubisco to enhance carbon fixation efficiency ( Sustainable land-use and ecosystem services A pivotal strategy to mitigate competition with food production and reduce emissions from indirect land-use change involves relocating tobacco cultivation to marginal or degraded lands ( Techno-economic feasibility and scaling Although the technical feasibility and environmental benefits of tobacco biorefineries appear promising, their commercial viability hinges on techno-economic feasibility

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