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Repeated amphetamine use, including Adderall, leads to psychological dependence, as users quickly build tolerance, requiring higher doses to achieve the same effects, which significantly increases addiction risk according to Knapp (1952) in the study Amphetamine and Addiction. The signs of Adderall addiction include increasing doses without medical guidance, obsessive thoughts about the drug, and difficulty reducing use despite personal, social, or physical harm

The control experiment, consisting of untreated CA substrate, was included to ensure a clear comparison between the control and pretreated samples