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The alkaloid content of tobacco leaves varies greatly depending on the soil conditions, use of fertilizers, and the degree to which the plant is ripened.[] A number of phytosterols such as cholesterol, campesterols and alcohols, phenolics, chlorogenic acid, rutin, carboxylic acids, turpenes, polyphenols, aromatic hydrocarbons, aldehydes, ketones, amines, nitrites, N- and O-heterocyclic hydrocarbons, pesticides, and alkali nitrates have been detected.[] Toxic metals including mercury, lead, chromium, and other trace elements and several free amino are also present.[] Nicotine, the addictive substance, exists in two forms, acid (bound) and base (free)