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CRISPR-Cas9 KO in human tracheal basal cells Two CRISPR RNA (crRNA) guides targeting human FOXN4 , FOXI1 , or POU2F3 annealed with universal tracrRNA and complexed with Alt-R HiFi Cas9 nuclease (1:1.2 duplex:nuclease), were electroporated (3.1 M RNP) into PEP/RI expanded human tracheal basal cells (5 10 5 cells/transfection) with the Amaxa Nucleofector II (pulse code W-001)

More recently, in August 2019, the US Centers for Disease Control and Prevention (CDC) declared an outbreak of the e-cigarette or vaping product use-associated lung injury (EVALI) which caused several deaths in young population (reviewed in [20])

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