However, it should be noted that the average nicotine strength of the consumed e-liquids according to the questionnaire can be considered as low (2.9 4.1 mg nicotine per ml), no data about the daily consume of e-cigarettes were available and Group 3 was heterogeneous (regular/occasional hookah consumers with or without regular/occasional vaping)
Cell culture Caco-2 epithelial colon cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and expanded in Dulbeccos modified Eagles medium (DMEM) supplemented with 20% FBS (Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) to promote optimal growth and viability
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You can design barriers to keep contamination from entering a room