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To enhance reflexivity and minimize bias, all field investigators took systematic field notes immediately after each interview to capture their contextual perspectives, observations, and initial reflections

The effects of E-liquid on MAP kinase signaling were examined by performing Western blots for the following targets: p-Erk, p p-38, p-JNK, p38, JNK, ERK and Actin

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In DSS-induced colitis, flow cytometry revealed significantly fewer Treg cells among the mononuclear cells isolated from the PBMCs, MLNs, and LPMCs of Gpr15 / mice compared with those of Gpr15 +/+ mice (Fig