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NLRP3-ASC inflammasome is involved in nicotine-induced EC pyroptosis Increases in the protein levels of cleaved caspase-1 (Casp1 p20) and mature IL-1 (17 kDa) and mature IL-18 (18 kDa) are the hallmarks of NLRP3 inflammasome activation 19

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Acknowledgements The authors would like to thank the financial support provided by the Mashhad University of Medical Sciences (Iran) through the grant number of 4021298

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