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Severe emphysema is classified as confluent (coalescence of centrilobular lucencies) or advanced destructive emphysema (ADE) if the expansion of the entire secondary lobule, distortion of the pulmonary architecture, and splaying or decreased caliber of vessels are present (Fig

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Overall, whether exposures are conducted via NO or WB exposure systems, the TPM concentration resulting from the smoke or aerosol generation must be measured in the breathing zone of the test animals or inside the exposure chamber and recorded, along with the temperature and humidity