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Subsequently, the membranes were incubated overnight at 4 o C with the primary antibody, followed by a 1-h incubation with the secondary antibody

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Someone once asked me..."What is your biggest personal burden?" "I smoke cigarettes." "Quit!" "I don't want to yet." "Why not?" "They relax me." "But, Annette - how can your biggest burden be relaxing?" Blank stare......"I don't know" In the end, all quitting amounted to was putting them in the dumpster and never picking them back up

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