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Immunoblotting To obtain total protein lysates, the cells or lung tissues were homogenized using radioimmunoprecipitation assay buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 1 mM ethylene diamine tetraacetic acid, 1 mM ethylene glycol tetraacetic acid, 1 mM Na 3 VO 4 , 20 mM NaF, 0.5 mM dl-dithiothreitol, 1 mM phenylmethane-sulfonyl fluoride, and a protease inhibitor cocktail in PBS

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*When printed without the white plate, the finish will be transparent and light-colored designs will be difficult to see

Taken together, e-cigarette use did not seem to work as a tool for tobacco smoking reduction or cessation on a population level