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Puff volume, inhalation depth, rate and intensity of puffing, the particle size distribution of the aerosol, nicotine concentration, and several physical states can all modify the concentrations of nicotine delivered 30 , 31

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The dominant microorganisms of CTLs play a major role in the degradation of aromatic compounds, hydrocarbons, aliphatic compounds, and lignin, and the structure of the microbial community composition varies from region to region, making cigar tobacco flavor highly variable

As the centuries passed, tobacco use in these societies became more varied