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RNA Extraction and RT-PCR RNA was extracted from 5 to 10 m thick sections of paraffin-embedded heart septum tissue using the High Pure RNA Paraffin Kit (Roche, Basel, Switzerland)

Tilt head forwards again and remove eye bath, discarding any remaining eye wash

Iron chelators, including ICL670, 311, and DFO, have demonstrated the ability to suppress HIV-1 replication by inhibiting viral transcription and activation (Salhi et al., 1998